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堿式氯化銅相對硫酸銅更穩(wěn)定，利用率更高，對環(huán)境排放污染少等諸多優(yōu)勢，已經(jīng)被行業(yè)普遍接受?，F(xiàn)階段堿式氯化銅在畜禽動物飼料中應(yīng)用已經(jīng)十分普遍，但是在水產(chǎn)動物中的應(yīng)用研究相對十分缺乏。而這是一篇關(guān)于堿式氯化銅在水產(chǎn)動物中的應(yīng)用研究文章，并提出了合理添加使用建議。個人覺得很有參考價值，并且今后有必要對其他的水產(chǎn)動物進(jìn)行堿式氯化銅的應(yīng)用研究。

A study was conducted to investigate the effects of dietary tribasic copper chloride on growth, copper status, antioxidant activities, immune responses and intestinal microflora in blunt snout bream, Megalobrama amblycephala. Eight practical diets containing different levels of copper (0, 3, 6, 9, 25, 50, 100 and 150 mg Cu kg− 1) from tribasic copper chloride were fed to blunt snout bream (31 g of initial average body weight) in quadruplicate groups in outdoor cages. Growth, copper status, antioxidant activities, immune responses and intestinal microflora were analyzed after a 56-day feeding period. Weight gain and specific growth rates in fish fed diets supplemented with 3–6 mg Cu kg− 1 were significantly higher than those in fish fed the control diet (which supplemented with 0 mg Cu kg− 1) (p < 0.05). Fish fed diets supplemented with 9 mg Cu kg− 1 had significantly lower feed conversion ratio than fish fed the control diets (p < 0.05). Copper concentrations in liver, kidney and intestine were significantly higher in fish fed diets with 50–150 mg Cu kg− 1 than those in fish fed the control diet (p < 0.05). Dietary copper at levels ranging from 9 mg kg− 1 to 100 mg kg− 1 significantly enhanced T-AOC activities (p < 0.05). In addition, diets supplemented with 100–150 mg Cu kg− 1 significantly increased activities of AKP and ACP (p < 0.05).Intestinal bacterial community structures in fish fed diets supplemented with 6–150 mg Cu kg− 1 were different from those in fish fed diets supplemented with 0–3 mg Cu kg− 1. The study showed that diets supplemented with 3–6 mg Cu kg− 1 could meet the copper requirements for blunt snout bream fed practical diets; diets supplemented with 9 mg Cu kg− 1 could increase feed efficiency; diets supplemented with 100 mg Cu kg− 1 could increase activities of AKP and ACP and affect intestinal microflora structure, though leading to a status of increasing copper accumulation in tissues.

本試驗旨在研究日糧中堿式氯化銅對團(tuán)頭魴生長、銅狀況、抗氧化活性、免疫應(yīng)答和腸道菌群的影響。8組實用日糧，分別添加了不同水平的堿式氯化銅(0, 3, 6, 9, 25, 50, 100 and 150 mg Cu kg−1) 來飼喂（初始重31g）團(tuán)頭魴，每組四個重復(fù)，露天桶養(yǎng)。飼喂56天后，對團(tuán)頭魴生長、銅狀況、抗氧化活性、免疫應(yīng)答和腸道菌群進(jìn)行分析。3-6mg Cu kg− 1 日糧組團(tuán)頭魴增重和特定生長率顯著高于對照組（添加0 mg Cu kg− 1） (p < 0.05)。9 mg Cu kg− 1 日糧組團(tuán)頭魴餌料系數(shù)顯著低于對照組(p < 0.05)。飼喂50–150 mg Cu kg− 1日糧組團(tuán)頭魴銅在肝臟、腎臟和腸道蓄積顯著高于對照組(p < 0.05)。銅添加水平從9 mg kg− 1 到 100 mg kg− 1 ，均顯著提高T-AOC活力(p < 0.05)。此外，添加100–150 mg Cu kg− 1日糧組顯著提高了AKP和ACP活力(p < 0.05)。6–150 mg Cu kg− 1 日糧組團(tuán)頭魴腸道菌群結(jié)構(gòu)與0–3 mg Cu kg− 1日糧組有差異。研究顯示，在日糧中添加3–6 mg Cu kg− 1 可以滿足團(tuán)頭魴對實用日糧銅的需求；在日糧中添加9 mg Cu kg− 1 能提高飼料效率；在日糧中添加100 mg Cu kg− 1雖然增加了組織的銅蓄積，但是還可以提高AKP和ACP活力，影響腸道菌群結(jié)構(gòu)。

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